| 1 |  Friuli ASD (Giovanni Bortoluzzi, Davide De Cassan, Marco Andreaus, Alberto Bruttomesso, Bryan Olivo) | 4:32 |
| 2 |  Team Ecoflo Chronos (Matisse Julien, Nicolas Rivard, Florian Carpentier, Lenaïc Langella, Félix Hamel) | 0:05 |
| 3 |  Equipe nationale d'Allemagne (Tobias Buck-Gramcko, Max Märkl, Moritz Kretschy, Vincent John, Moritz Czasa) | 00:07 |
| 4 | Green Project-Bardiani Csf-Faizanè (Alessandro Pinarello, Alessio Martinelli, Giulio Pellizzari, Matteo Scalco, Luca Paletti) | 0:07 |
| 5 |  Tirol (Tim Wafler, Paul Buschek, Sebastian Putz, Moritz Hörandtner, Linus Rosner) | 0:08 |
| 6 |  Tartu2024 Cycling Team (Lauri Tamm, Aaron Aus, Alekss Krasts, Jomantas Venckus, Markus Knaapi) | 0:09 |
| 7 |  JEGG - DJR Academy (Stef Koning, Kacper Gieryk, Marijn Maas, Tjalle de Bruin, Sven Pijnenburg) | 0:11 |
| 8 | Scorpions Racing Team (Hugo Kars, Wessel Mouris, Jasper Huitema, Niek Voogt, Nathan Székely) | 0:12 |
| 9 |  Tudor Pro Cycling Team U23 (Tim Rey, Elia Blum, Aivaras Mikutis, Zachary Walker, Robin Donzé) | 0:12 |
| 10 | Sensa - Kanjers Voor Kanjers (Bram Leerkes, Mart Van Hoorn, Eskil Huiting, Maarten Hamminga, James Potter) | 0:12 |
| 11 |  Elkov - Kasper (Tomás Pridal, Tomás Obdrzálek, Jan Lukes, Daniel Mráz, Filip Reha) | 0:12 |
| 12 |  Klub Kolarski Tarnovia (Radoslaw Fratczak, Piotr Maslak, Jakub Musialik, Julian Kot, Antoni Kida) | 0:12 |
| 13 | GKS Cartusia Kartuzy (Kacper Majewski, Maciej Nocen, Jakub Soszka, Mateusz Cywinski, Szymon Pomian) | 0:12 |
| 14 |  Team Ibt - Tripeak (Marckus Njor, Peter Asbjørn Hansen, Samuel Mackenzie Hodge, Kasper Due Kaspersen, Isak Magnusson) | 0:13 |
| 15 |  Bingoal - WB Development Team (Matteo Melotte, Tom Portsmouth, Theo Lowie, Sil Van Daele, Noah Detalle) | 0:14 |
| 16 | Q36.5 Continental Team (Hector Ferney Molina, Ephrem Hebrehiwet, Nahom Zerai, Hamza Amari, Travis Stedman) | 0:17 |
| 17 |  Equipe nationale de Slovénie (Dan Andrej Tomsic, Primoz Kirbis, Gal Glivar, Jernej Hribar, Vid Jeromel) | 0:20 |
| 18 | Bialini Gomola Ccn (George Radcliffe, Daniel Felipe Orjuela, Eric Inkster, Daniel Yon Hin, Alejandro Jerson Paez) | 0:20 |
| 19 |  Cycling Academy Trencín (Lukás Sokolík, Jakub Vlcák, Samuel Kovác, Adam Gross) | 0:22 |
| 20 | Equipe nationale de Slovaquie (Denis Hoza, Jakub Galovic, Filip Lohinský, Christian Haas, Martin Jurík) | 0:23 |
| 21 | Lubelskie Perla Polski (Robert Kendzia, Lukasz Juszczak, Mikolaj Szulik, Pawel Wroblewski, Karol Wawrzyczek) | 0:24 |
| 22 |  Equipe nationale d'Ukraine (Yurii Shcherban, Sviatoslav Horbovyi, Valentyn Kabashnyi, Mykyta Yakovlev, Oleksander Smetaniuk) | 0:25 |
| 23 |  Lotus Team (ákos Mindszenti, Luka Dimitrijoski, Levente Vaskó, Jovan Divnic, Mark Percic) | 0:26 |
| 24 | Mostostal Pulawy (Jakub Zurek, Karol Kusy, Kacper Bartkowski, Filip Biernacki, Bartlomiej Kasperek) | 0:28 |
| 25 |  MKB Bank Cycling Team (Dániel Sidló, Bálint Orosz, Benedek Hlavathy, Zsombor Juhász) | 0:30 |
